Humpty Dumpty Circus (lost animated short; 1908)
From The Lost Media Wiki
A still from the short.
Status: Lost
The Humpty Dumpty Circus was a stop motion short made in 1898 that was directed by J. Stuart Blackton and produced by Albert E. Smith, who also used his daughter's dolls as props. It's considered to be the first (known) stop motion short ever made, but has unfortunately been lost to time with no footage available, and only some screenshots of it remain. Considering how old it is combined with the lack of preservation in the early 1900's and below, it's highly unlikely that it will ever be found.